Journal: Journal of Neurophysiology

Article Title: Adenosine inhibits glutamatergic input to basal forebrain cholinergic neurons

doi: 10.1152/jn.00528.2011

Figure Lengend Snippet: Cell counting in BF nuclei of neurons labeled by Cy3-p75 NTR -IgG and positive for ChAT immunoreactivity

Article Snippet: More recently, a new polyclonal fluorescent antibody against murine p75 NTR (Cy3-p75 NTR -IgG; Advanced Targeting Systems) has become available.

Techniques: Cell Counting, Labeling

Journal: Journal of Neurophysiology

Article Title: Adenosine inhibits glutamatergic input to basal forebrain cholinergic neurons

doi: 10.1152/jn.00528.2011

Figure Lengend Snippet: Cy3-coupled antibodies raised against mouse p75 neurotrophin receptors (Cy3-p75NTR-IgGs) specifically label cholinergic neurons in the basal forebrain (BF). Photographs show neurons of the horizontal limb of the diagonal band (hDB), magnocellular preoptic nucleus (MCPO), and substantia innominata (SI) nuclei. p75NTR-IgG label is shown in red and choline acetyltransferase (ChAT) immunoreactivity in green. A: ChAT immunostaining in the hDB shown at low magnification (left; scale bar = 100 μm). ChAT-positive neurons and p75NTR-IgG-positive neurons (boxed area) are shown at higher magnification at middle and right, respectively (scale bars = 25 μm). B: ChAT immunostaining in the MCPO and SI at the level of the crossing fibers of the anterior commissure is shown at low magnification (top row, left; scale bar = 200 μm). ChAT-positive neurons and p75NTR-IgG-positive neurons of the MCPO (bottom boxed area) and of the SI (top boxed area) are displayed at higher magnification at top row, middle and right, for MCPO and at bottom row, left and middle, for SI (scale bars = 25 μm).

Article Snippet: More recently, a new polyclonal fluorescent antibody against murine p75 NTR (Cy3-p75 NTR -IgG; Advanced Targeting Systems) has become available.

Techniques: Immunostaining

Journal: Oncotarget

Article Title: The p75 neurotrophin receptor regulates cranial irradiation-induced hippocampus-dependent cognitive dysfunction

doi: 10.18632/oncotarget.16492

Figure Lengend Snippet: Western blot analysis of p75 NTR in the hippocampus (A) and PFC (B) at 1, 2 and 3 months post-irradiation. Right: Representative immunoblots. Quantitative analysis reveals an increase mean integrated optical density of p75 NTR in the DG region at 2 months post-irradiation (C) . Representative confocal microscopy images (low magnification) showing the expression pattern of p75 NTR in rat hippocampus (D) . Magnified images (right) show colocalization between p75 NTR and NeuN in normal rat hippocampus (D-a) and irradiated rat hippocampus (D-b), p75 NTR and GFAP (D-c) in the DG region. Data are presented as mean ±SEM. * p <0.05; ** p <0.01; *** p <0.001. n=5/group.

Article Snippet: For knockdown p75 NTR , shRNA oligomers targeting mouse p75 NTR were purchased from Genechem (Shanghai, China).

Techniques: Western Blot, Irradiation, Confocal Microscopy, Expressing

Journal: Oncotarget

Article Title: The p75 neurotrophin receptor regulates cranial irradiation-induced hippocampus-dependent cognitive dysfunction

doi: 10.18632/oncotarget.16492

Figure Lengend Snippet: (A-C) Morris water maze test . Comparison of the escape latencies (A), mean swimming speed (B) and the percentage of target quadrant exploring time in probe test (C) between the AAV-GFP and AAV-P75 groups. (D-F) Object location recognition test . Diagram of the object location recognition task (D); The graph shows the object exploration during the test phase (E); Both AAV-GFP and AAV-p75 rats spent more time exploring a novel object location (F). (G-I) Novel object recognition test . Diagram of the object recognition task (G); The graph shows the object exploration during the 5 min test phase (H); The AAV-p75 rats did not display any preference for an object placed to a novel object (I). (J-K) Open field test . No significant differences were detected in total distance travelled test (J), and percent time travelled in the centre of the open files (K) between AAV-GFP and AAV-p75 groups. All data are presented as mean ±SEM. * p <0.05; ** p <0.01. n= 10-15/group.

Article Snippet: For knockdown p75 NTR , shRNA oligomers targeting mouse p75 NTR were purchased from Genechem (Shanghai, China).

Techniques: Comparison

Journal: Oncotarget

Article Title: The p75 neurotrophin receptor regulates cranial irradiation-induced hippocampus-dependent cognitive dysfunction

doi: 10.18632/oncotarget.16492

Figure Lengend Snippet: (A) The levels of p75 NTR in hippocampus extracts from AAV-ctl, AAV-irradiation and AAV-shp75 rats after virus-injection 1 month were detected. Right: Representative immunoblots. (B-E) Morris Water Maze test . Escape latencies (B), average swimming speeds (C) the percentage of target quadrant exploring time (D), and representative images of swimming paths (E) are shown. (F) Object location recognition test . No significant differences were detected in the times spent exploring a novel location among groups. (G) Novel object recognition test . AAV-irradiation rats showed worse retention performance than AAV-ctl rats. (H-I) Open field test . No significant differences were detected in the mean distance (H), and the percent time in the centre (I) among groups. All histograms represent mean ± SEM. *p< 0.05; **p< 0.01. n=15-20/group.

Article Snippet: For knockdown p75 NTR , shRNA oligomers targeting mouse p75 NTR were purchased from Genechem (Shanghai, China).

Techniques: Irradiation, Virus, Injection, Western Blot

Journal: Foods

Article Title: Increased Levels of Phosphorylated-P38α Induce WNT/β-Catenin and NGF/P75NTR/TrkA Pathways Disruption and SN56 Cell Death following Single and Repeated Chlorpyrifos Treatment

doi: 10.3390/foods13152427

Figure Lengend Snippet: Analysis of CPF action on p-P38α ( A ), P75 NTR ( B ), and PAI-1 ( C ) levels. Results are shown as a percentage of control values. Results of protein content were normalized by total protein concentrations. *** p ≤ 0.001 compared to control. &&& p ≤ 0.001 compared to P38α -silenced cells exposed to CPF.

Article Snippet: SN56 cells were transfected using siRNA (Qiagen, Barcelona, Spain) homologous to mouse P75 NTR (GS18053), and P38α (GS26416) target genes following the HiPerfect Transfection reagent guideline.

Techniques: Control

Journal: Foods

Article Title: Increased Levels of Phosphorylated-P38α Induce WNT/β-Catenin and NGF/P75NTR/TrkA Pathways Disruption and SN56 Cell Death following Single and Repeated Chlorpyrifos Treatment

doi: 10.3390/foods13152427

Figure Lengend Snippet: Results from cell viability (MTT assay) ( A ) and apoptosis (caspase 3/7 activity assay) ( B ). Results were normalized by total protein concentrations. Results are shown as a percentage of control values. *** p ≤ 0.001 compared to control. ### p ≤ 0.001 compared to CPF treatment. &&& p ≤ 0.001 compared to P75 NTR -silenced cells treated with CPF. γγγ p ≤ 0.001 compared to rβ-Catenin and CPF treatment. τττ p ≤ 0.001 compared to P38α -silenced cells treated with CPF.

Article Snippet: SN56 cells were transfected using siRNA (Qiagen, Barcelona, Spain) homologous to mouse P75 NTR (GS18053), and P38α (GS26416) target genes following the HiPerfect Transfection reagent guideline.

Techniques: MTT Assay, Activity Assay, Control

Journal: PLoS ONE

Article Title: Fibroblasts Isolated from Human Middle Turbinate Mucosa Cause Neural Progenitor Cells to Differentiate into Glial Lineage Cells

doi: 10.1371/journal.pone.0076926

Figure Lengend Snippet: Two different morphologies of OECs were found. Both types were both GFAP and p75 NTR positive labeled. One type (A–D) with filamentous GFAP labeling (B) and punctate p75 NTR labeling (C). Arrow head identifies one cell with single p75 NTR labeling. Cells labeled only with p75 NTR were not counted as OECs in this study. Another type (E, F) had intense p75 NTR labeling (red) and diffuse GFAP labeling (green). Scale bars: 50 µm.

Article Snippet: The following primary antibodies were used: mouse anti-human Thy1.1 (1∶1200, Serotec), rabbit anti-human basic fibroblast growth factor (FGF2, 1∶100, Santa Cruz Biotechnology), mouse anti-human Nestin (1∶200, Chemicon), mouse anti-human chondroitin sulfate proteoglycan (CSPG, 1∶100, Chemicon), mouse anti-human fibronectin (1∶100, Chemicon), rabbit anti-glial fibrillary acidic protein (GFAP, 1∶250, DAKO), mouse anti-human low affinity neurotrophin receptor p75 (p75 NTR , 1∶750, USBiological), and mouse anti-β Tubulin III (anti-Tubulin, 1∶75, Sigma).

Techniques: Labeling

Journal: PLoS ONE

Article Title: Fibroblasts Isolated from Human Middle Turbinate Mucosa Cause Neural Progenitor Cells to Differentiate into Glial Lineage Cells

doi: 10.1371/journal.pone.0076926

Figure Lengend Snippet: The percentage of p75 NTR and GFAP positive cells in the total cells (A) from middle turbinates of 7 patients. Representative photographs of middle turbinate tissue: patient 1 (B) and patient 6 (C). In C, bone was separated from lamina propria. Note that the size of samples from two patients was similar, but the yields of OECs were very different.

Article Snippet: The following primary antibodies were used: mouse anti-human Thy1.1 (1∶1200, Serotec), rabbit anti-human basic fibroblast growth factor (FGF2, 1∶100, Santa Cruz Biotechnology), mouse anti-human Nestin (1∶200, Chemicon), mouse anti-human chondroitin sulfate proteoglycan (CSPG, 1∶100, Chemicon), mouse anti-human fibronectin (1∶100, Chemicon), rabbit anti-glial fibrillary acidic protein (GFAP, 1∶250, DAKO), mouse anti-human low affinity neurotrophin receptor p75 (p75 NTR , 1∶750, USBiological), and mouse anti-β Tubulin III (anti-Tubulin, 1∶75, Sigma).

Techniques: